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 >  Protein>TNF-alpha >TNA-H5255

Human TNF-alpha Protein, Fc Tag (MALS verified)

分子别名(Synonym)

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

表达区间及表达系统(Source)

Human TNF-alpha Protein, Fc Tag (TNA-H5255) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).

Predicted N-terminus: Val 77

Request for sequence

蛋白结构(Molecular Characterization)

TNF-alpha Structure

This protein carries a human IgG1 Fc tag at the C-terminus

The protein has a calculated MW of 43.8 kDa. The protein migrates as 50-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

TNF-alpha SDS-PAGE

Human TNF-alpha Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

TNF-alpha SEC-MALS

The purity of Human TNF-alpha Protein, Fc Tag (Cat. No. TNA-H5255) is more than 90% and the molecular weight of this protein is around 255-285 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

TNF-alpha ELISA

Immobilized Human TNFR1, His Tag (Cat. No. TN1-H5222) at 5 μg/mL (100 μL/well) can bind Human TNF-alpha Protein, Fc Tag (Cat. No. TNA-H5255) with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

 

背景(Background)

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimers disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

 

前沿进展

 
 
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TNF-alpha靶点信息
英文全称:Tumor necrosis factor α
中文全称:肿瘤坏死因子α
种类:Homo sapiens
上市药物数量:61详情
临床药物数量:63详情
最高研发阶段:批准上市
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