膜杰作
Star Staining
分子别名（Synonym）
NGF,NGFB,Beta-NGF,HSAN5,MGC161426,MGC161428
表达区间及表达系统（Source）
Human Beta-NGF Protein, premium grade (BEF-H5214) is expressed from human 293 cells (HEK293). It contains AA Ser 122 - Ala 241 (Accession # P01138).
Predicted N-terminus: Ser 122
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
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蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 13.5 kDa. The protein migrates as 14-15 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
无菌（Sterility）
The sterility testing was performed by membrane filtration method.
支原体（Mycoplasma）
Negative.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 20 mM NaAC, 150 mM NaCl, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Beta-NGF Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human Beta-NGF Protein, premium grade (Cat. No. BEF-H5214) is more than 90% and the molecular weight of this protein is around 32-50 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-Bioactivity CELL BASE
Human Beta-NGF Protein, premium grade (Cat. No. BEF-H5214) stimulates proliferation of TF-1 cells. The EC50 for this effect is 2.963 ng/mL (QC tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Human Beta-NGF Protein, premium grade (Cat. No. BEF-H5214) at 1 μg/mL (100 μL/well) can bind Human TrkA (33-417), Mouse IgG2a Fc Tag (Cat. No. TRA-H5259) with a linear range of 1-16 ng/mL (QC tested).
Protocol
Immobilized Human Beta-NGF Protein, premium grade (Cat. No. BEF-H5214) at 1 μg/mL (100 μL/well) can bind Human TrkA (192-402), Mouse IgG2a Fc Tag (Cat. No. TRA-H5253) with a linear range of 1-16 ng/mL (Routinely tested).
Protocol
背景（Background）
Nerve growth factor beta (beta-NGF) is a neurotrophic factor that plays critical role in the development and maintenance of sensory and sympathetic neurons. The active form of human beta-NGF is is a noncovalently disulfide-linked homodimer, which is secreted and signals through the low affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase A (TrkA) to activate PI3K, Ras, and PLC signaling pathways.
