膜杰作
Star Staining
抗体来源（Source）
HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with IgG.
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with IgG.
应用（Application）
ELISA,WB
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS,pH 7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 6 months after reconstitution;
- 2-8°C for 2-3 weeks under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228) at 2 μg/mL (100 μL/well) can bind Human Monoclonal Anti-TNF-alpha antibody, Human IgG1 (16H5) (Cat. No. TNA-AM494) when detected by HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8),mAb (Cat. No. IGG-LY69) dilute at 1:10000 (0.0842μg/ml) (QC tested).
Protocol
背景（Background）
Immunoglobulins can be divided into five main classes/isotypes which are IgA, IgD, IgE, IgG, and IgM. IgG class identity is determined by class-specific sequences in the Fc region of the heavy chain. IgG antibody class are the most abundant immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4). The IgG subclasses differ in their physical and chemical properties. Their distribution pattern is found to be age dependent and every subclass has a specific biological function.
