|Standard Curve Range||19.5 pg/mL-1250 pg/mL|
|Assay Time||2 hr 50 min|
|Suitable Sample Type||For the quantitative determination of human TNF-α in Cell Culture Supernatants, Plasma, Serum.|
|Sample volume||100 μL|
|CRS002-C01||Pre-coated Anti-TNF-α Antibody Microplate||1plate|
|CRS002-C02||Human TNF-α Standard||20μg|
|CRS002-C05||10xWashing Buffer||50 mL|
|CRS002-C06||2xDilution Buffer||50 mL|
|CRS002-C07||Substrate Solution||12 mL|
|CRS002-C08||Stop Solution||7 mL|
Tumour Necrosis Factor alpha (TNF-α) ELISA Kit is based on ELISA sandwich method and designed to measure human TNF-α levels in cell culture supernates, serum, and plasma. It contains recombinant human TNF-α and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human TNF-α concentrations. The specificity has been verified.
The kit is developed for quantitative detection of TNF-α in human serum and cell culture supernates.
It is for research use only.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储 & 运输（Storage & Shipping）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.
Below is the Shipping Statement for Kit Products.
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human TNF-α. The kit consists of Pre-coated Anti-TNF-α Antibody Microplate and Human TNF-α Standard and Biotin-Anti-TNF-α Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20°C-25°C) before use.
b) Add your sample to the plate and take the Human TNF-α as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-TNF-α Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
典型数据-Typical Data Please refer to DS document for The assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The following CRS-A002 standard curve data is for reference only.
DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα
Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimers disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.