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Virus-Like Particle (VLP) isotype control

分子别名(Synonym)

VLP,Virus Like Particle,eVLP,VLPs

表达区间及表达系统(Source)

VLP isotype control (VLP-N5213) is expressed from human 293 cells (HEK293).

蛋白结构(Molecular Characterization)

VLP isotype control (VLP-N5213) is formed by self-assembly of envelop/capsid proteins from viruses, it can be used as isoytype control for VLPs displaying membrane proteins in various applications.

Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrance Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

内毒素(Endotoxin)

Less than 0.5 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

均一性(Identity)-DLS

Identity DLS

The mean peak Radius of VLP is 60-80 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

背景(Background)

Virus-like particles (VLPs) are highly structured protein particles composed of single or multiple structural proteins of the virus. Its morphological structure is similar to that of natural virus particles, does not contain virus accounting, and has strong immunogenicity and biological activity. The empty shell structure of most VLP is icosahedral or helical structure, which is composed of several specific structural proteins. At present, it is widely used in vaccine production and antigen presentation.

 

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