膜杰作
Star Staining
分子别名（Synonym）
GSK3B,Glycogen synthase kinase-3 beta
表达区间及表达系统（Source）
Human GSK-3beta, His Tag (GSB-H5545) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Thr 420 (Accession # P49841-1).
Predicted N-terminus: Met 1
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus
The protein has a calculated MW of 48.8 kDa. The protein migrates as 49-53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Human GSK-3beta, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-HPLC
The purity of Human GSK-3beta, His Tag (Cat. No. GSB-H5545) was greater than 90% as determined by SEC-HPLC.
活性（Bioactivity）
The specific activity of GSK-3beta was determined to be >120 pmol/min/ug by ADP-Glo™ Kinase Assay using a synthetic substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE) (QC tested).
Protocol
背景（Background）
Glycogen synthase kinase-3 (GSK-3) is a pivotal molecule in the development of Alzheimer's disease (AD). GSK-3beta is involved in the formation of paired helical filament (PHF)-tau, which is an integral component of the neurofibrillary tangle (NFT) deposits that disrupt neuronal function, and a marker of neurodegeneration in AD.
