膜杰作
Star Staining
分子别名（Synonym）
CD158f
表达区间及表达系统（Source）
Human KIR2DL5 / CD158f Protein, Fc Tag (KIA-H5255) is expressed from human 293 cells (HEK293). It contains AA His 22 - His 240 (Accession # Q8N109-1).
Predicted N-terminus: His 22
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 50 kDa. The protein migrates as 63-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine,25 mM Arg,150 mM NaCl,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human KIR2DL5 / CD158f Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-SPR
Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Complex Protein (Cat. No. HLM-H82E4) immobilized on CM5 Chip can bind Human KIR2DL5 / CD158f Protein, Fc Tag (Cat. No. KIA-H5255) with an affinity constant of 27.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
Killer cell immunoglobulin-like receptor 2DL5(KIR2DL5), which belongs to the immunoglobulin superfamily, is an inhibitory receptor of NK cells. KIR2DL5 is encoded by two paralogous genes displaying copy number variation and allelic polymorphism-KIR2DL5A and KIR2DL5B that vary by only 2 aa in domain and 1 aa in signal peptide. KIR2DL5 and KIR2DL4 form a subfamily, which have longer cytoplasmic tails than other KIR, and each has one D0 and one D2-type Ig-like domain.
