C-C motif chemokine 14,Chemokine CC-1/CC-3,HCC-1/HCC-3,HCC-1(1-74),NCC-2,Small-inducible cytokine A14,CCL14,NCC2, SCYA14
Human CCL14, His Tag (CC4-H5249) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Asn 93 (Accession # Q16627-1).
Predicted N-terminus: His
Request for sequence
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 10.7 kDa. The protein migrates as 14-15 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human CCL14, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
CCL14 has weak activity on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca2+ changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophil