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 >  生物标记素蛋白>FcRn (FCGRT & B2M) >FCM-R82W6

Biotinylated Rabbit FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™&Tag Free (MALS & SPR verified)

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分子别名(Synonym)

FcRn,FCGRT & B2M

表达区间及表达系统(Source)

Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (FCM-R82W6) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Val 297 (FCGRT) & Val 22 - Tyr 120 (B2M) (Accession # ABY47597.1(FCGRT) & ALR85722.1(B2M)).

Predicted N-terminus: Ala 24 (FCGRT) & Val 22 (B2M)

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 24 - Val 297 (FCGRT) & Val 22 - Tyr 120 (B2M)) ABY47597.1 (FCGRT) & ALR85722.1 (B2M)

Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 33.8 kDa (FCGRT) & 11.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 35-40 kDa (FCGRT) & 12 kDa (B2M) respectively due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-R82W6) SDS-PAGE gel

Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-R82W6) MALS images

The purity of Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-R82W6) is more than 90% and the molecular weight of this protein is around 42-62 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-R82W6) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin® with an affinity constant of 1.05 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-R82W6) on SA Chip can bind Herceptin® with an affinity constant of 1.53 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

 

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