膜杰作
Star Staining
分子别名（Synonym）
folate receptor 3 (gamma),Folate receptor 3,folate receptor gamma,FOLR3,FR-G,FR-gamma,gamma-Hfr,
表达区间及表达系统（Source）
Human FOLR3, His Tag (FO3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Ser 245 (Accession # P41439-1).
Predicted N-terminus: Gln 23
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 27.4 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human FOLR3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human FOLR3, His Tag (Cat. No. FO3-H52H3) is more than 90% and the molecular weight of this protein is around 28-42 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Folic acid-BSA immobilized on CM5 Chip can bind Human FOLR3, His Tag (Cat. No. FO3-H52H3) with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
背景（Background）
FOLR3, also know as folate receptor gamma, is a member of FRs. Folic acid and its reduced derivatives are transported via two widely expressed transporters, the reduced folate carrier (RFC) and the proton-coupled folate transporter (PCFT), and via a family of glycosyl-phosphatidylinositol (GPI)-anchored receptors with limited expression profiles known as folate receptors (FRs).Folate receptors (FR) are high affinity receptors that transport folate via endocytosis. Human FR exists in three isoforms, alpha and beta equipped with GPI anchors, and gamma not possessing a GPI anchor.
