膜杰作
Star Staining
产品描述（Product Details）
Laminin 511 E8 (LN511-E8) is a recombinant human protein that provides a defined surface for in vitro feeder-free culture of multiple human pluripotent stem cells (PSCs). Being a truncated form of laminin 511, LN511-E8 serves as a functionally minimal form that retain the full capability for binding to integrins. LN511-E8 has been proven to maintain normal growth characteristics and stemness in multiple PSC lines without simultaneous differentiation, which includes ESC, iPSC, MSC etc. In addition, LN511-E8 has been demonstrated to support PSC growth for >10 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. As published by Takamichi Miyazaki et al. , the LN511-E8 variant of laminin 511 shows higher efficiency for supporting the adhesion of dissociated cells than did wild-type laminin 511 which makes a cost-effective choice.
Optimized and defined surface
Laminin 511 E8 (LN511-E8) has been proved to allow single-cell seeding at low density. In a feeder-free culture system, the seeded cells demonstrate high motility with higher clonal survival.
Physiology-related
The laminin 511 isoform is crucial to the growth and maintenance of hPSCs in human through its binding to cell receptors a6B1 integrin. LN511-E8 is truncated laminin 511 isoform which works in multiple stem cell lines, from iPSC to hESC and MSC.
Better Adhesion
LN511-E8 variant of laminin 511 shows higher efficiency for supporting the adhesion of dissociated cells than did wild-type laminin 511.
Cost Effective
LN511-E8 maintains pluripotency at a lower dose without simultaneous differentiation when compared to laminin 521. Hence LN511-E8 provides a cost-effective option for feeder free culture of human pluripotent stem cells (PSCs).
Reduce Variability
LN511-E8 is a defined, recombinant human protein with better lot-to-lot consistency that reduces variability in your PSC cultures.
产品参数（Key parameter）
| Purity (SDS PAGE) | > 95% |
| Mycoplasma Test | Negative |
| Sterility Test | Negative |
| Integrin Binding Assay | ≤ 10 nM |
| Endotoxin Test | < 0.01 EU per μg |
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-Stem Cell CultureView Protocol
Laminin 511 (LA8-H5283) could maintain the stemness of iPSC at least Passage 5. Immunofluorescent staining indicated that the iPSCs expressed high levels of pluripotency associated markers Sox2, TRA-1-60, SSEA4 and OCT4.
Laminin 511 (LA8-H5283) could maintain the stemness of iPSC at least Passage 5. FACS data indicated that the iPSCs expressed high levels of pluripotency associated markers OCT4 and SOX2.
Normal karyotype (46, XX) was found in hiPSCs with Laminin 511 (LA8-H5283) coating after 10 passages.
活性（Bioactivity）-BLI
Loaded Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) on Protein A Biosensor, can bind Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wc) with an affinity constant of 3.82 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) on Protein A Biosensor, can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
