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 >  经MALS验证的蛋白>IL-2 R beta & IL-2 R gamma >ILG-H82F3

Biotinylated Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc,Avitag™&Fc,Avitag™ (MALS verified)

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分子别名(Synonym)

IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG

表达区间及表达系统(Source)

Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag (ILG-H82F3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG) (Accession # P14784-1 (IL-2RB) & P31785-1 (IL-2RG)).

Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)

Request for sequence

蛋白结构(Molecular Characterization)

Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 52.8 kDa (IL-2RB) and 55.3 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The reducing (R) protein migrates as 60-66 kDa and 80-90 kDa respectively due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-2 R beta & IL-2 R gamma SDS-PAGE

Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

IL-2 R beta & IL-2 R gamma ELISA

Immobilized Human IL-2, Tag Free (Cat. No. IL2-H4113) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F3) with a linear range of 0.01-0.156 μg/mL (QC tested).

Protocol

IL-2 R beta & IL-2 R gamma ELISA

Immobilized Human IL-15, premium grade (Cat. No. IL5-H4117) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F3) with a linear range of 0.039-0.625 μg/mL (Routinely tested).

Protocol

背景(Background)

Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

 

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