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SARS-CoV-2 Nucleocapsid Protein-coupled Magnetic Beads

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Coupled amount of protein>1000 pmol (49 μg) Nucleocapsid protein/mg beads1000 pmol (49 μg) Nucleocapsid protein/mg beads
Capacity>130 pmol (20 μg) Anti-Nucleocapsid antibody/mg beads>130 pmol (20 μg) Anti-Nucleocapsid antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Product FormatPowderPowder
Reconstitution2 mL ultrapure water (1 mg beads / mL)Reconstitute 5 mg / bottle Beads with 5 mL ultrapure water (1 mg beads / mL)
Concentration1 mg beads / mL1 mg beads / mL

Background/背景

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Application/应用

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

Reconstitution/重构方法

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

Upon receipt, please store the Beads at -20℃ for 1 year in lyophilized state.

Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

Assay Principles/原理

The Antigen pre-coupled magnetic beads are coupled with biotinylated protein onto streptavidin (SA) magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. We provide the SARS-CoV-2 nucleocapsid protein-coupled magnetic beads, which could help you to capture the antibody, and easily to follow up with other tests, such as immunocapture, biopanning and flow cytometry.

Application Method:

a) Reconstitute the Beads following the CoA, wash the Beads and suspended to a certain concentration by adding assay Buffer.

b) Add your antibody to the beads, incubation and wash the beads.

 

Typical Data/典型数据

Biotinylated Human ELISA

Immobilized 49 μg SARS-CoV-2 Nucleocapsid protein to 1 mg Beads can bind the Anti-SARS-CoV-2 Nucleocapsid Antibody with an EC50 of 0.6712 μg/mL (QC tested).

Protocol

前沿进展

 
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