|Items||Size （2mg）||Size（5mg X 2）|
|Particle size||2 μm||2 μm|
|Coupled amount of protein||>1000 pmol (49 μg) Nucleocapsid protein/mg beads||1000 pmol (49 μg) Nucleocapsid protein/mg beads|
|Capacity||>130 pmol (20 μg) Anti-Nucleocapsid antibody/mg beads||>130 pmol (20 μg) Anti-Nucleocapsid antibody/mg beads|
|Formulation||PBS, pH7.4, with 10% Trehalose||PBS, pH7.4, with 10% Trehalose|
|Reconstitution||2 mL ultrapure water (1 mg beads / mL)||Reconstitute 5 mg / bottle Beads with 5 mL ultrapure water (1 mg beads / mL)|
|Concentration||1 mg beads / mL||1 mg beads / mL|
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Upon receipt, please store the Beads at -20℃ for 1 year in lyophilized state.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
The Antigen pre-coupled magnetic beads are coupled with biotinylated protein onto streptavidin (SA) magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. We provide the SARS-CoV-2 nucleocapsid protein-coupled magnetic beads, which could help you to capture the antibody, and easily to follow up with other tests, such as immunocapture, biopanning and flow cytometry.
a) Reconstitute the Beads following the CoA, wash the Beads and suspended to a certain concentration by adding assay Buffer.
b) Add your antibody to the beads, incubation and wash the beads.
Immobilized 49 μg SARS-CoV-2 Nucleocapsid protein to 1 mg Beads can bind the Anti-SARS-CoV-2 Nucleocapsid Antibody with an EC50 of 0.6712 μg/mL (QC tested).