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Biotinylated Human Transferrin R / CD71 Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

CD71,TFRC,TFR,sTfR,TfR1,Trfr,T9,p90

表达区间及表达系统(Source)

Biotinylated Human Transferrin R, His,Avitag (TFR-H82E5) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 760 (Accession # P02786-1 ).

Predicted N-terminus: Gly

Request for sequence

蛋白结构(Molecular Characterization)

Transferrin R Structure

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 78.7 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Transferrin R SDS-PAGE

Biotinylated Human Transferrin R, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Transferrin R SEC-MALS

The purity of Biotinylated Human Transferrin R, His,Avitag (Cat. No. TFR-H82E5) is more than 90% and the molecular weight of this protein is around 160-180 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Transferrin R ELISA

Immobilized Human Transferrin Protein, His Tag (Cat. No. TRN-H52H3) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R, His,Avitag (Cat. No. TFR-H82E5) with a linear range of 1.2-9.7 ng/mL (QC tested).

Protocol

 

背景(Background)

CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.

 

前沿进展

 
 
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Transferrin R靶点信息
英文全称:Transferrin receptor protein 1
中文全称:转铁蛋白受体1
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:9详情
最高研发阶段:批准上市
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