Recombinant Proteins iso9001 ISO13485
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Human IL-23 R Protein, His Tag (SPR verified)

分子别名(Synonym)

IL-23 R,IL-23 Receptor

表达区间及表达系统(Source)

Human IL-23 R, His Tag (SPR verified) (ILR-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Gly 355 (Accession # Q5VWK5-1).

Predicted N-terminus: Gly 24

蛋白结构(Molecular Characterization)

Online(Gly 24 - Gly 355) Q5VWK5-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 39.9 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-23 R, His Tag (SPR verified) (Cat. No. ILR-H52H4) SDS-PAGE gel

Human IL-23 R, His Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-H82W6) captured on Biotin CAP - Series S sensor Chip can bind Human IL-23 R, His Tag (SPR verified) (Cat. No. ILR-H52H4) affinity constant of 3.39 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-H82W6) on SA Biosensor, can bind Human IL-23 R, His Tag (SPR verified) (Cat. No. ILR-H52H4) with an affinity constant of 33.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin 23 receptor (IL-23R) is a type I cytokine receptor, and IL-23R pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. Also, IL-23R associates constitutively with Janus kinase 2 (JAK2), and binds to transcription activator STAT3 in a ligand-dependent manner. Furthermore, IL-23R mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. As for IL-23, it may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.

 

前沿进展

 
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IL-23R靶点信息
英文全称:Interleukin 23 Receptor
中文全称:白细胞介素23受体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:批准上市
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