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Human Nectin-2 / CD112 Protein, His Tag

分子别名(Synonym)

CD112,Nectin-2,PVRL2

表达区间及表达系统(Source)

Human Nectin-2, His Tag (PV2-H52E2) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).

Predicted N-terminus: Gln 32

蛋白结构(Molecular Characterization)

Online(Gln 32 - Leu 360) Q92692-2

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 37.4 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Nectin-2, His Tag (Cat. No. PV2-H52E2) SDS-PAGE gel

Human Nectin-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human PVRIG, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PVG-H5253) at 2 μg/mL (100 μL/well) can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with a linear range of 0.078-0.625 μg/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human TIGIT, Fc Tag (Cat. No. TIT-H5254) at 10 μg/mL (100 μL/well) can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with a linear range of 0.625-5 μg/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Human PVRIG, Fc Tag (Cat. No. PVG-H5257) at 5 μg/mL (100 μL/well) can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with a linear range of 0.039-0.625 μg/mL (Routinely tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human PVRIG, Fc Tag (Cat. No. PVG-H5257) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.61 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Rat TIGIT, Fc Tag (Cat. No. TIT-R5258) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.59 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.66 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

 

前沿进展

 
货号/价格

PV2-H52E2-100ug / ¥3000

PV2-H52E2-1mg / ¥18000

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