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Human B7-H3 / CD276 Protein, Fc Tag

分子别名(Synonym)

B7-H3,CD276,B7 homolog 3

表达区间及表达系统(Source)

Human B7-H3, Fc Tag (B73-H5253) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2).

Predicted N-terminus: Leu 29

蛋白结构(Molecular Characterization)

Online(Leu 29 - Pro 245) Q5ZPR3-2

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 50 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human B7-H3, Fc Tag (Cat. No. B73-H5253) SDS-PAGE gel

Human B7-H3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human B7-H3, Fc Tag (Cat. No. B73-H5253) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Protocol

文献引用(Citations)

背景(Background)

B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.

 

前沿进展

货号/价格

B73-H5253-100ug / ¥2900

B73-H5253-1mg / ¥18000

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B7-H3靶点信息
英文全称:CD276 antigen
中文全称:CD276抗原
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:6详情
最高研发阶段:临床二期
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