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Biotinylated Human CD200 / OX-2 Protein, Fc,Avitag™

分子别名(Synonym)

CD200,MOX1,MOX2,MRC,OX-2,My033

表达区间及表达系统(Source)

Biotinylated Human CD200, Fc,Avitag (OX2-H82F1) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gly 232 (Accession # P41217-1).

Predicted N-terminus: Gln 31

蛋白结构(Molecular Characterization)

Online(Gln 31 - Gly 232) P41217-1

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 51.4 kDa. The protein migrates as 65-76 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CD200, Fc,Avitag (Cat. No. OX2-H82F1) SDS-PAGE gel

Biotinylated Human CD200, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD200 R1, His Tag (Cat. No. CR2-H52H6) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD200, Fc,Avitag (Cat. No. OX2-H82F1) with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

背景(Background)

CD200 is also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.

 

前沿进展

 
货号/价格

OX2-H82F1-25ug / ¥3500

OX2-H82F1-200ug / ¥13800

开发建议
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400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
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order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
CD200靶点信息
英文全称:OX-2 membrane glycoprotein
中文全称:OX-2膜糖蛋白
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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