Cynomolgus LAG-3, Fc Tag (LA3-C5252) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - His 449 (Accession # XP_005570011.1).
Predicted N-terminus: Ala 18
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 73.0 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>85% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus LAG-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
Immobilized Cynomolgus LAG-3, Fc Tag (Cat. No. LA3-C5252) at 2 μg/mL (100 μL/well) can bind Anti-Human LAG3 MAb, Human IgG1 with a linear range of 8-31 ng/mL (QC tested).
Flow Cytometry assay shows that Cynomolgus LAG-3, Fc Tag (Cat. No. LA3-C5252) can bind to daudi cell surface. The concentration of LAG-3 used is 1 μg/ml (Routinely tested).
Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.