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Biotinylated Human Mesothelin / MSLN (296-580) Protein, His,Avitag™ (MALS verified)

分子别名(Synonym)

MSLN,Mesothelin,MPF

表达区间及表达系统(Source)

Biotinylated Human Mesothelin (296-580), His,Avitag (MSN-H82E9) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).

Predicted N-terminus: Glu 296

蛋白结构(Molecular Characterization)

Online(Glu 296 - Gly 580) AAH09272.1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 35.9 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human Mesothelin (296-580), His,Avitag (Cat. No. MSN-H82E9) SDS-PAGE gel

Biotinylated Human Mesothelin (296-580), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human Mesothelin (296-580), His,Avitag (Cat. No. MSN-H82E9) MALS images

The purity of Biotinylated Human Mesothelin (296-580), His,Avitag(Cat. No. MSN-H82E9) was more than 90% and the molecular weight of this protein is around 45-55 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-Human MSLN MAb at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin (296-580), His,Avitag (Cat. No. MSN-H82E9) with a linear range of 0.1-0.78 ng/mL (QC tested).

Protocol

CAR阳性表达率检测(Evaluation of CAR expression )

FACS Analysis of Anti-MSLN CAR Expression

CAR

293 cells were transfected with Anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Mesothelin (296-580), His,Avitag (Cat. No. MSN-H82E9, 3 µg/mL) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (Anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human Mesothelin (296-580), His,Avitag (Cat. No. MSN-H82E9).

Protocol

 

背景(Background)

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

 

前沿进展

 
货号/价格

MSN-H82E9-25ug / ¥2500

MSN-H82E9-200ug / ¥9800

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Mesothelin靶点信息
英文全称:Mesothelin
中文全称:间皮素
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:15详情
最高研发阶段:临床二期
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