Mouse TFR2, Mouse Fc Tag (TF2-M5269) is expressed from human 293 cells (HEK293). It contains AA Arg 103 - Phe 798 (Accession # Q9JKX3-1).
Predicted N-terminus: Val
This protein carries a mouse IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 101.1 kDa. The protein migrates as 116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 0.1 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Mouse TFR2, Mouse Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Authors: Stocki P, Szary J, Rasmussen CLM, et al.
Journal: FASEB journal 2020
Transferrin receptor protein 2 (TFR2) is a single-pass type II membrane protein with a protease associated (PA) domain, an M28 peptidase domain and a transferrin receptor-like dimerization domain. The mutations in TFR2 gene have been associated with hereditary hemochromatosis type III. Furthermore, TFR2 is involved in the uptake of transferrin-bound iron into cells by endocytosis, although its role is minor compared to transferrin receptor 1.