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Biotinylated Human TNF-alpha Protein, epitope tag free, ultra sensitivity (primary amine labeling) (active trimer) (MALS verified)

分子别名(Synonym)

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

表达区间及表达系统(Source)

MABSol® Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (TNA-H8211) is expressed from human HEK293 cells. It contains AA Val 77 - Leu 233 (Accession # NP_000585.2). It is the biotinylated form of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (TNA-H4211).

Predicted N-terminus: Val 77

蛋白结构(Molecular Characterization)

Online(Val 77 - Leu 233) NP_000585.2

The product does NOT contain any epitope tags. The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa on a SDS-PAGE gel under reducing (R) condition.

标记(Biotinylation)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 1-3 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by reduced SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (Cat. No. TNA-H8211) SDS-PAGE gel

Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (Cat. No. TNA-H8211) MALS images

The purity of Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (Cat. No. TNA-H8211) was more than 95% in HP-SEC, and the molecular weight of this protein is around 48-65 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (Cat. No. TNA-H8211) with a linear range of 0.4-6 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (Cat. No. TNA-H8211) with a linear range of 0.4-6 ng/mL (Routinely tested).

Protocol

文献引用(Citations)

背景(Background)

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

 

前沿进展

 
货号/价格

TNA-H8211-25ug / ¥2900

TNA-H8211-200ug / ¥12500

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
TNF-alpha靶点信息
英文全称:Tumor necrosis factor α
中文全称:肿瘤坏死因子α
种类:Homo sapiens
上市药物数量:46详情
临床药物数量:126详情
最高研发阶段:批准上市
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