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Human TIM-3 / HAVCR2 (22-200) Protein, Fc Tag

分子别名(Synonym)

HAVCR2,TIM3,TIMD3,FLJ14428,KIM3

表达区间及表达系统(Source)

Human TIM-3, Fc Tag (TM3-H5258) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Arg 200 (Accession # Q8TDQ0-1).

Predicted N-terminus: Ser 22

蛋白结构(Molecular Characterization)

Online(Ser 22 - Arg 200) Q8TDQ0-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 46.5 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>85% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human TIM-3, Fc Tag (Cat. No. TM3-H5258) SDS-PAGE gel

Human TIM-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human TIM-3, Fc Tag (Cat. No. TM3-H5258) at 2 μg/mL (100 μL/well) can bind Anti-TIM3 MAb with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

背景(Background)

Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428,  KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

 

前沿进展

 
货号/价格

TM3-H5258-100ug / ¥3500

TM3-H5258-1mg (500ug X 2) / ¥19800

开发建议
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TIM-3靶点信息
英文全称:Hepatitis A virus cellular receptor 2
中文全称:甲型肝炎病毒细胞受体2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:12详情
最高研发阶段:临床三期
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