Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
SARS S protein RBD, His Tag (SPD-S52H6) is expressed from human 293 cells (HEK293). It contains AA Arg 306 - Phe 527 (Accession # AAP13567.1).
Predicted N-terminus: Arg 306
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.9 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
SARS S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) was more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.
Immobilized SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-4 ng/mL (QC tested).
Immobilized SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (Routinely tested).
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) with an affinity constant of 17.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.