Spike,S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1,COVID-19
SARS S1 protein, His Tag (S1N-S52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Arg 667 (Accession # AAP13567.1).
Predicted N-terminus: Ser 14
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 74.9 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
SARS S1 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS S1 protein, His Tag (Cat. No. S1N-S52H5) was more than 85% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
Immobilized SARS S1 protein, His Tag (Cat. No. S1N-S52H5) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-8 ng/mL (QC tested).
Immobilized SARS S1 protein, His Tag (Cat. No. S1N-S52H5) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-6 ng/mL (Routinely tested).
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS S1 protein, His Tag (Cat. No. S1N-S52H5) with an affinity constant of 36.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Kondo T, Iwatani Y, Matsuoka K, et al.
Journal: Science advances 2020
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.