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Cynomolgus Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI & HPLC verified)

分子别名(Synonym)

CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2

表达区间及表达系统(Source)

Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (CDA-C52H7) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Pro 208 (Accession # Q8SPW4-1).

Predicted N-terminus: Gln 28

蛋白结构(Molecular Characterization)

Online(Gln 28 - Pro 208) Q8SPW4-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.1 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by reduced SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) SDS-PAGE gel

Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC

Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) HPLC images

The purity of Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) was greater than 90% as determined by SEC-HPLC.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Captured Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) on CM5 Chip via Anti-His antibody can bind Rituximab with an affinity constant of 4.18 μM as determined in SPR assay (Biacore T200) (QC tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 3.3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Rituximab on FAB2G Biosensor, can bind Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) with an affinity constant of 6.0 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

 

前沿进展

 
货号/价格

CDA-C52H7-50ug / ¥2200

CDA-C52H7-100ug / ¥3000

CDA-C52H7-1mg / ¥19800

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