Human Her2, Tag Free (HE2-H5212) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
Predicted N-terminus: Thr 23
This protein carries no "tag".
The protein has a calculated MW of 70.1 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Her2, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Her2, Tag Free (Cat. No. HE2-H5212) at 1 μg/mL (100 μL/well)can bind Trastuzumab with a linear range of 0.2-2 ng/mL (QC tested).
Loaded Hercecptin on AHC Biosensor, can bind Human Her2, Tag Free (Cat. No. HE2-H5212) with an affinity constant of 1.45 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Turner, Brendan L et al.
Journal: Analytical chemistry 2020
Application: Dual-Affinity Ratiometric Quenching (DARQ) Assay
Authors: Hutterer KM, et al.
Journal: BioDrugs 43565
Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .