Human 4-1BB, Fc Tag (41B-H5258) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # NP_001552.2).
Predicted N-terminus: Leu 24
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 43.4 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human 4-1BB, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) at 0.1 μg/mL (100 μL/well) can bind Biotinylated Human 4‑1BB Ligand, Fc Tag, Avi Tag (Cat. No. 41L-H82F9) with a linear range of 0.4-13 ng/mL (QC tested).
Immobilized Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) at 1 μg/mL (100 μL/well) can bind Anti-Human 4-1BB MAb, Human IgG4 with a linear range of 0.2-3 ng/mL (Routinely tested).
Loaded Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) on Protein A Biosensor, can bind Human 4-1BB Ligand, His Tag (Cat. No. 41L-H5249) with an affinity constant of 1.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Flow Cytometry assay shows that Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0.1 μg/mL (Routinely tested).
Authors: V Brucklacher-waldert, C Edwards, J Legg.
Journal: US20200362047A1 2020
Application: Binding Assay
Authors: P Bobrowicz, et al
Journal: US20190060454A1 2019
4-1BB is also known as CD137, tumor necrosis factor receptor superfamily member 9 (TNFRSF9), induced by lymphocyte activation (ILA), is a co-stimulatory molecule of the tumor necrosis factor (TNF) receptor superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. CD137 can enhance activation-induced T cell apoptosis when triggered by engagement of the TCR/CD3 complex. In addition, 4-1BB/4-1BBL co-stimulatory pathway has been shown to augment secondary CTL responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF2.