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Rhesus macaque Growth Hormone R (GHR) Protein, Fc Tag

分子别名(Synonym)

GHR,GHBP,GH receptor

表达区间及表达系统(Source)

Rhesus macaque GHR, Fc Tag (GHR-C5253) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Tyr 264 (Accession # P79194-1).

Predicted N-terminus: Phe 19

蛋白结构(Molecular Characterization)

Online(Phe 19 - Tyr 264) P79194-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 54.7 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Rhesus macaque GHR, Fc Tag (Cat. No. GHR-C5253) SDS-PAGE gel

Rhesus macaque GHR, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human GH, Tag Free at 2 μg/mL (100 μL/well) can bind Rhesus macaque GHR, Fc Tag (Cat. No. GHR-C5253) with a linear range of 0.039-0.625 μg/mL (QC tested).

Protocol

背景(Background)

Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

 

前沿进展

 
货号/价格

GHR-C5253-100ug / ¥3500

GHR-C5253-1mg (500ug X 2) / ¥22000

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Growth Hormone R靶点信息
英文全称:Growth hormone receptor
中文全称:生长激素受体
种类:Homo sapiens
上市药物数量:24详情
临床药物数量:45详情
最高研发阶段:批准上市
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