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Biotinylated Human IL-17A&IL-17F Protein, Avitag™,His Tag

分子别名(Synonym)

IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,Interleukin-17F,Cytokine ML-1

表达区间及表达系统(Source)

Biotinylated Human IL-17A&IL-17F, Avitag,His Tag (ILF-H82W1) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F) (Accession # Q16552-1(IL-17A) & Q96PD4-1(IL-17F)).

Predicted N-terminus: Gly 24

蛋白结构(Molecular Characterization)

Online(Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F)) Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)

Biotinylated Human IL-17A&IL-17F, Avitag,His Tag, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 34.1 kDa. Subunit IL-17A&IL-17F is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The reducing (R) protein migrates as 40-43 kDa due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human IL-17A&IL-17F, Avitag,His Tag (Cat. No. ILF-H82W1) SDS-PAGE gel

Biotinylated Human IL-17A&IL-17F, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-Human IL17A MAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17A&IL-17F, Avitag,His Tag (Cat. No. ILF-H82W1) with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human IL-17A&IL-17F, Avitag,His Tag (Cat. No. ILF-H82W1) on SA Biosensor, can bind Human IL-17 RA, His Tag (Cat. No. ILA-H5222) with an affinity constant of 352 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Biotinylated Human IL-17A&IL-17F, Avitag,His Tag (Cat. No. ILF-H82W1) on SA Biosensor, can bind uman IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 217 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

 

前沿进展

 
货号/价格

ILF-H82W1-25ug / ¥3500

ILF-H82W1-200ug / ¥13800

开发建议
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400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
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order.cn@acrobiosystems.com
技术支持邮箱:
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