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Mouse SIRP alpha / CD172a Protein, Fc Tag

分子别名(Synonym)

SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1

表达区间及表达系统(Source)

Mouse SIRP alpha, Fc Tag (SIA-M5258) is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # BAA13520.1).

Predicted N-terminus: Lys 32

蛋白结构(Molecular Characterization)

Online(Lys 32 - Asn 373) BAA13520.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 64.3 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse SIRP alpha, Fc Tag (Cat. No. SIA-M5258) SDS-PAGE gel

Mouse SIRP alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M525a) at 10 μg/mL (100 μL/well) can bind Mouse SIRP alpha, Fc Tag (Cat. No. SIA-M5258) with a linear range of 10-156 ng/ml (QC tested).

Protocol

Biotinylated Human  ELISA

Report

背景(Background)

Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

 

前沿进展

 
货号/价格

SIA-M5258-100ug / ¥3000

SIA-M5258-1mg / ¥19800

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