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Human PD-1 / PDCD1 Protein, Llama IgG2b Fc Tag, low endotoxin

分子别名(Synonym)

PDCD1,PD1,CD279,SLEB2

表达区间及表达系统(Source)

Human PD-1, Llama IgG2b Fc Tag, low endotoxin (PD1-H5259) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).

Predicted N-terminus: Leu 25

蛋白结构(Molecular Characterization)

Online(Leu 25 - Gln 167) Q15116-1

This protein carries a llama IgG2b Fc tag at the C-terminus.

The protein has a calculated MW of 43.8 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human PD-1, Llama IgG2b Fc Tag, low endotoxin (Cat. No. PD1-H5259) SDS-PAGE gel

Human PD-1, Llama IgG2b Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human PD-L1, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) at 0.5 μg/mL (100 μL/well) can bind Human PD-1, Llama IgG2b Fc Tag, low endotoxin (Cat. No. PD1-H5259) with a linear range of 20-313 ng/mL (QC tested).

点击申请Protocol

Biotinylated Human SPR

Immobilized Human PD-1, Llama IgG2b Fc Tag, low endotoxin (Cat. No. PD1-H5259) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-2 ng/mL (Routinely tested).

点击申请Protocol

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

背景(Background)

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

 

前沿进展

货号/价格

PD1-H5259-100ug / ¥3500

PD1-H5259-1mg (500ug X 2) / ¥23100

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PD-1靶点信息
英文全称:Programmed cell death protein 1
中文全称:细胞程序性死亡-1
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:38详情
最高研发阶段:批准上市
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