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Biotinylated Human ANGPTL7 / CDT6 Protein, Mouse IgG2a Fc,Avitag™

分子别名(Synonym)

ANGPTL7,CDT6

表达区间及表达系统(Source)

Biotinylated Human ANGPTL7, Mouse IgG2a Fc,Avitag (AN7-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Pro 346 (Accession # O43827-1).

Predicted N-terminus: Gln 27

蛋白结构(Molecular Characterization)

Online(Gln 27 - Pro 346) O43827-1

This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 65.8 kDa. The protein migrates as 66 kDa and 75-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human ANGPTL7, Mouse IgG2a Fc,Avitag (Cat. No. AN7-H82F3) SDS-PAGE gel

Biotinylated Human ANGPTL7, Mouse IgG2a Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human LILRB2, Fc Tag (Cat. No. CDD-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc,Avitag (Cat. No. AN7-H82F3) with a linear range of 0.156-2.5 μg/mL (QC tested).

Protocol

背景(Background)

Angiopoietin-like 7 (ANGPTL7) is also known as Corneal-Derived Transcript 6 (CDT6), and is a secreted glycoprotein that is structurally related to the angiopoietins. It is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells.

 

前沿进展

 
货号/价格

AN7-H82F3-25ug / ¥3500

AN7-H82F3-200ug (100ug X 2) / ¥13800

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