搜索
 >  免疫检查点蛋白>OX40 Ligand >Mouse OX40 Ligand / TNFSF4 Protein, His Tag

Mouse OX40 Ligand / TNFSF4 Protein, His Tag

分子别名(Synonym)

OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1

表达区间及表达系统(Source)

Mouse OX40 Ligand, His Tag (TN4-M5241) is expressed from human 293 cells (HEK293). It contains AA Gln 49 - Leu 198 (Accession # P43488-1).

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

Unconjugated(Gln 49 - Leu 198) P43488-1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 18.3 kDa. The protein migrates as 20 kDa and 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse OX40 Ligand, His Tag (Cat. No. TN4-M5241) SDS-PAGE gel

Mouse OX40 Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse OX40 Ligand, His Tag (Cat. No. TN4-M5241) at 0.5 μg/mL (100 μL/well) can bind Mouse OX40, Fc Tag (Cat. No. OX0-M5259) with a linear range of 10-16 ng/mL (QC tested).

点击申请Protocol

背景(Background)

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

 

前沿进展

货号/价格

TN4-M5241-50ug / ¥3700

TN4-M5241-500ug (100ug x 5) / ¥24400

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
OX40 Ligand靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 4
中文全称:OX40配体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
查看更多信息

消息提示

请输入您的联系方式,再点击提交!

确定