搜索
 >  其他蛋白>CD52 >CD2-H82F3

Biotinylated Human CD52 Protein, Fc,Avitag™

分子别名(Synonym)

CDW52,HE5,CD52,CAMPATH-1 antigen

表达区间及表达系统(Source)

Biotinylated Human CD52, Fc,Avitag (CD2-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ser 36 (Accession # P31358-1 ).

Predicted N-terminus: Gly 25

蛋白结构(Molecular Characterization)

Online(Gly 25 - Ser 36) P31358-1

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 29.7 kDa. As a result of glycosylation, the protein migrates as 38-45 kDa under reducing (R) condition, and 66-100 kDa under non-reducing (NR) condition (SDS-PAGE).

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CD52, Fc,Avitag (Cat. No. CD2-H82F3) SDS-PAGE gel

Biotinylated Human CD52, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-CD52 MAb, Human IgG1 at 10 μg/mL (100 μL/well) can bind Biotinylated Human CD52, Fc,Avitag (Cat. No. CD2-H82F3) with a linear range of 0.625-10 μg/mL (QC tested).

Protocol

背景(Background)

CAMPATH-1 antigen, also known as cluster of differentiation 52 (CD52), is a glycoprotein that in humans is encoded by the CD52 gene.It is widely expressed on the cell surface of immune cells, such as mature lymphocytes, natural killer cells (NK), eosinophils, neutrophils, monocytes/macrophages, and dendritic cells (DCs).ligation of cell surface CD52 molecules may offer costimulatory signals for T-cell activation and proliferation.

 

前沿进展

 
货号/价格

CD2-H82F3-25ug / ¥3500

CD2-H82F3-200ug / ¥13800

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
CD52靶点信息
英文全称:CAMPATH-1 antigen
中文全称:CAMPATH-1抗原
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:6详情
最高研发阶段:批准上市
查看更多信息

消息提示

请输入您的联系方式,再点击提交!

确定