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ActiveMax® Human TNF-alpha Protein, Tag Free, low endotoxin (active trimer) (MALS verified)

分子别名(Synonym)

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

表达区间及表达系统(Source)

ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (TNA-H4211) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).

Predicted N-terminus: Val 77

蛋白结构(Molecular Characterization)

Online(Val 77 - Leu 233) P01375-1

This protein carries no "tag".

The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa and 18 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 6 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) SDS-PAGE gel

ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) MALS images

The purity of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) was more than 95% in HP-SEC, and around 47-60 kDa verified by SEC-MALS.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.2-3 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014 ng/mL (Routinely tested).

Protocol

Biotinylated Human CELL_BASE

Neutralization assay shows that the cytotoxicity effect of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 29 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

 

前沿进展

货号/价格

TNA-H4211-25ug / ¥750

TNA-H4211-100ug / ¥1900

TNA-H4211-1mg (500ug X 2) / ¥10500

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
TNF-alpha靶点信息
英文全称:Tumor necrosis factor α
中文全称:肿瘤坏死因子α
种类:Homo sapiens
上市药物数量:45详情
临床药物数量:127详情
最高研发阶段:批准上市
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