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Monoclonal Anti-Monkeypox-E8L Antibody, Mouse IgG1 (7B8)

抗体来源(Source)

Monoclonal Anti-Monkeypox-E8L Antibody, Mouse IgG1 (7B8) antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with E8L.

亚型(Isotype)

Mouse IgG1 | Mouse Kappa

特异性(Specificity)

This product is a specific antibody specifically reacts with E8L.

应用(Application)

ELISA,FITC

纯度(Purity)

>95% as determined by SDS-PAGE.

内毒素(Endotoxin)

Less than 1.0 EU per mg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS,pH 7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

E8L SDS-PAGE

Monoclonal Anti-Monkeypox-E8L Antibody, Mouse IgG1 (7B8) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

E8L ELISA

Immobilized Monkeypox virus (strain Zaire-96-I-16) E8L Protein, His Tag (Cat. No. E8L-M52H3) at 2μg/mL (100μL/well) can bind Monoclonal Anti-Monkeypox -E8L Antibody, Mouse IgG1 (7B8) (Cat. No. E8L-Y87) with a linear range of 0.04-100 ng/mL (QC tested).

Protocol

背景(Background)

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded D. The cases are mostly in central and western Africa. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa , multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. E8L can Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.

 

前沿进展

 
 
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