| ID | Components | Size | Size |
| A010-214 | SARS-CoV-2 S protein RBD | 10 μg | 60 μg |
| A011-214 | Biotinylated Human ACE2 | 10 μg | 10 μg |
| A003-214 | Streptavidin-HRP | 10 μg | 10 μg |
| AC2-NA005 | SARS-CoV-2 Inhibitor (Human ACE2) | 20 μg | 50 μg |
Background/背景
The newly identified 2019 novel coronavirus (SARS-CoV-2) has posed a serious threat to human health. Currently, there is no specific treatment for COVID-19. It is urgent to develop the SARS-CoV-2 inhibitors, for example vaccines and therapeutic antibodies and small molecular compounds against COVID-19.
Application/应用
This kit is useful for screening inhibitors of SARS-CoV-2.
ELISA Buffer Set-96T (EBS-001) is sold separately and not included in kit, and please contact us if you need the buffer.
Reconstitution/重构方法
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage/存储
Upon receipt, please store all items at -20℃ to -70℃. After reconstitution, the stock solution should be kept at -70℃.
It is recommended not to freeze thaw more than 3 times.
This product is stable after storage at: Room temperature (RT) for 1 month in lyophilized state; -20℃ to -70℃ for 1 year in lyophilized state; -70℃ for 6 months under sterile conditions after reconstitution
Assay Principles/原理
This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of SARS-CoV-2 inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized SARS-CoV-2 S protein RBD and in-house developed biotinylated Human ACE2 protein. This product is uniquely suitable for rapid high-throughput screening of SARS-CoV-2 inhibitors. Briefly, we provide you with a biotinylated Human ACE2 protein, a SARS-CoV-2 S protein RBD protein, an SARS-CoV-2 inhibitor (as method verified Reference), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with SARS-CoV-2 S protein RBD.
b) Add 50ul biotinylated human ACE2 to coated plate.
c) Add the reference or your molecule of interest.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit S protein RBD: ACE2 binding will be determined by comparing OD readings among different experimental groups.
Typical Data/典型数据
Binding of Biotinylated Human ACE2 To Immobilized SARS-CoV-2 S protein RBD In A Functional ELISA Assay.
Immobilized 2019-CoV-2 S protein RBD at 0.5 μg/mL (100 μL/well) can bind human ACE2-Biotin with a linear range of 0.001- 0.06 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting.
Protocol
Inhibition of Human ACE2 [Biotinylated] :SARS-CoV-2 S protein RBD Binding by SARS-CoV-2 inhibitor.
Serial dilutions of SARS-CoV-2 inhibitor (Cat. No. AC2-NA005) (1:2 serial dilutions, from 20 μg/mL to 0.039 μg/mL (180 nM to 0.35 nM)) was added into Biotinylated ACE2: SARS-CoV-2 S protein RBD binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting.
Protocol
文献引用（Citations）
-
Authors: Balkrishna, Acharya et al.
Journal: Drug Des Devel Ther 2021
Application: ELISA
