|A010-214||SARS-CoV-2 S protein RBD||10 μg||60 μg|
|A011-214||Biotinylated Human ACE2||10 μg||10 μg|
|A003-214||Streptavidin-HRP||10 μg||10 μg|
|AC2-NA005||SARS-CoV-2 Inhibitor (Human ACE2)||20 μg||50 μg|
The newly identified 2019 novel coronavirus (SARS-CoV-2) has posed a serious threat to human health. Currently, there is no specific treatment for COVID-19. It is urgent to develop the SARS-CoV-2 inhibitors, for example vaccines and therapeutic antibodies and small molecular compounds against COVID-19.
This kit is useful for screening inhibitors of SARS-CoV-2.
ELISA Buffer Set-96T (EBS-001) is sold separately and not included in kit, and please contact us if you need the buffer.
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Upon receipt, please store all items at -20℃ to -70℃. After reconstitution, the stock solution should be kept at -70℃.
It is recommended not to freeze thaw more than 3 times.
This product is stable after storage at: Room temperature (RT) for 1 month in lyophilized state; -20℃ to -70℃ for 1 year in lyophilized state; -70℃ for 6 months under sterile conditions after reconstitution
This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of SARS-CoV-2 inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized SARS-CoV-2 S protein RBD and in-house developed biotinylated Human ACE2 protein. This product is uniquely suitable for rapid high-throughput screening of SARS-CoV-2 inhibitors. Briefly, we provide you with a biotinylated Human ACE2 protein, a SARS-CoV-2 S protein RBD protein, an SARS-CoV-2 inhibitor (as method verified Reference), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with SARS-CoV-2 S protein RBD.
b) Add 50ul biotinylated human ACE2 to coated plate.
c) Add the reference or your molecule of interest.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit S protein RBD: ACE2 binding will be determined by comparing OD readings among different experimental groups.
Binding of Biotinylated Human ACE2 To Immobilized SARS-CoV-2 S protein RBD In A Functional ELISA Assay.
Immobilized 2019-CoV-2 S protein RBD at 0.5 μg/mL (100 μL/well) can bind human ACE2-Biotin with a linear range of 0.001- 0.06 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting.
Inhibition of Human ACE2 [Biotinylated] :SARS-CoV-2 S protein RBD Binding by SARS-CoV-2 inhibitor.
Serial dilutions of SARS-CoV-2 inhibitor (Cat. No. AC2-NA005) (1:2 serial dilutions, from 20 μg/mL to 0.039 μg/mL (180 nM to 0.35 nM)) was added into Biotinylated ACE2: SARS-CoV-2 S protein RBD binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting.
Authors: Balkrishna, Acharya et al.
Journal: Drug Des Devel Ther 2021