Human 4-1BB Ligand, His Tag (41L-H5249) is expressed from human 293 cells (HEK293). It contains AA Ala 50 - Glu 254 (Accession # P41273-1).
Predicted N-terminus: Ala 50
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.3 kDa. The protein migrates as 25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human 4-1BB Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. 41B-H5256) at 1 μg/mL (100 μL/well) can bind Human 4‑1BB Ligand, His Tag (Cat. No. 41L-H5249) with a linear range of 0.1-2 ng/mL (QC tested).
Loaded Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) on Protein A Biosensor, can bind Human 4-1BB Ligand, His Tag (Cat. No. 41L-H5249) with an affinity constant of 1.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.