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SARS-CoV-2全系列产品助力新冠病毒攻坚战

蛋白纯度高，天然构象完整

货号：SPN-C52H9 产品：SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)

The well-folded trimeric particles of SARS-CoV-2 S protein, His Tag, Super stable trimer (SPN-C52H9) was verified by negative stain electron micrography. The particles are similar in size and appearance to SARS-CoV-2 trimers reported in published literature.
天然结合活性经验证
活性（Bioactivity）-ELISA

货号：S1N-C5257
产品：SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) with a linear range of 0.4-6 ng/mL (QC tested).

Protocol

活性（Bioactivity）-FACS

货号：S1N-C5257
产品：SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag

FACS analysis shows that SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) can bind to Vero E6 cells surface ACE2. The concentration of SARS-CoV-2 S1 protein is 3 μg/ml. (Routinely tested).

Protocol

宿主受体与病毒蛋白结合验证 (ELISA&BLI)
活性（Bioactivity）- ELISA

货号：AC2-H5257
产品：Human ACE2 / ACEH Protein, Fc Tag (MALS verified)

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 0.4-6 ng/mL (QC tested).

Protocol

活性（Bioactivity）- BLI

货号：NR1-H82E3
产品：Biotinylated Human Neuropilin-1 / NRP1 / CD304 Protein, His,Avitag™

Loaded Biotinylated Human Neuropilin-1, His,Avitag (Cat. No. NR1-H82E3) on SA Biosensor, can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H3) with an affinity constant of 0.663 μM as determined in BLI assay (ForteBio Octet Red96e)

Protocol

抗体

抗体结合活性经验证
货号：SPD-M128
产品：Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128)

活性（Bioactivity）- ELISA

Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H1) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128) (Cat. No. SPD-M128) with a linear range of 0.2-1.5ng/mL (QC tested).

Protocol

货号：SAD-S35
产品：Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35)

活性（Bioactivity）- ELISA

FACS analysis shows that the binding of SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5259) to Vero E6 cells surface ACE2 was inhibited by increasing concentration of Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35). The concentration of SARS-CoV-2 S protein RBD used is 5μg/ml. The IC50 is 10.33 μg/ml (Routinely tested).

Protocol

活性（Bioactivity）- FACS

FACS analysis shows that SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5259) can bind to Vero E6 cells surface ACE2. The concentration of SARS-CoV-2 S protein RBD is 5 μg/ml.

Protocol

中和抗体中和活性经假病毒验证

货号：SPD-M128 产品：Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128)

Serial dilutions of Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb (Cat. No. SPD-M128) were incubated with luciferase/SARS-CoV-2 spike pseudoviruses. HEK293T cells stably expressing ACE2 were added after 90 min and neutralization titers (IC50) is 0.03255 nM, which is calculated as the serum dilution at which RLU were reduced by 50% compared with RLU in virus control wells after subtraction of background RLU in cell control wells.

Protocol

中和抗体与RBD蛋白及其突变体亲和力经验证

货号：SPD-M128 产品：Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128)

中和抗体与RBD蛋白及其突变体亲和力比较（BLI）（KD: M）

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