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分子别名（Synonym）
Flt-4, FLT4, LMPH1A, PCLFLT41, VEGFR3, VEGFR-3, FLT-4, FLT-41, FLT41, PCL
表达区间及表达系统（Source）
Mouse VEGF R3 Protein, Mouse IgG2a Fc Tag (FL4-M5251) is expressed from human 293 cells (HEK293). It contains AA Tyr 25 - Asp 770 (Accession # P35917-1).
Predicted N-terminus: Tyr 25
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 111.5 kDa. The protein migrates as 116-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Mouse VEGF R3 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) at 5 μg/mL (100 μL/well) can bind Mouse VEGF R3 Protein, Mouse IgG2a Fc Tag (Cat. No. FL4-M5251) with a linear range of 0.31-5 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Mouse VEGF-D Protein, Fc Tag (Cat. No. VED-M5253) captured on CM5 chip via anti-Human IgG Fc antibody, can bind Mouse VEGF R3 Protein, Mouse IgG2a Fc Tag (Cat. No. FL4-M5251) with an affinity constant of 6.69 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Mouse VEGF R3 Protein, Mouse IgG2a Fc Tag (Cat. No. FL4-M5251) captured on CM5 chip via anti-mouse antibodies surface can bind Biotinylated Mouse VEGF-D Protein, His,Avitag (Cat. No. VED-M82E4) with an affinity constant of 2.13 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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