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分子别名（Synonym）
Flt-4,FLT4,LMPH1A,PCLFLT41,VEGFR3,VEGFR-3,FLT-4,FLT-41,FLT41,PCL
表达区间及表达系统（Source）
Human VEGF R3, Fc Tag (FL4-H5251) is expressed from human 293 cells (HEK293). It contains AA Tyr 25 - Ile 776 (Accession # NP_002011.2).
Predicted N-terminus: Tyr 25
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 111.2 kDa. The protein migrates as 140-160 kDa under reducing (R) condition, and 280-320 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human VEGF R3, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) is more than 85% and the molecular weight of this protein is around 260-320 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) at 5 μg/mL (100 μL/well) can bind Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5μg/mL (100 μL/well) can bind Human VEGF-D, His Tag (Cat. No. VED-H5228) with a linear range of 0.08-0.6 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag (Cat. No. VED-H5228) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) capture on NTA-Series S sensor chip can bind Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) with an affinity constant of 0.130 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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