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分子别名（Synonym）
CD71, TFRC, TFR, sTfR, TfR1, Trfr, T9, p90
表达区间及表达系统（Source）
Cynomolgus Transferrin R Protein, Fc Tag (TFR-C5263) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 760 (Accession # G8F602-1).
Predicted N-terminus: Pro
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 101.6 kDa. The protein migrates as 95-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Cynomolgus Transferrin R Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus Transferrin R Protein, Fc Tag (Cat. No. TFR-C5263) is more than 90% and the molecular weight of this protein is around 195-240 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Transferrin Protein, His Tag (Cat. No. TRN-H52H3) at 5 μg/mL (100 μL/well) can bind Cynomolgus Transferrin R Protein, Fc Tag (Cat. No. TFR-C5263) with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Cynomolgus Transferrin R Protein, Fc Tag (Cat. No. TFR-C5263) captured on Protein A Chip can bind Rhesus macaque Transferrin Protein, His Tag (Cat. No. TRN-R52H3) with an affinity constant of 0.795 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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