Human TRAIL R4 / TNFRSF10D Protein, Fc Tag (MALS verified)

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TR4-H5253-100ug
¥3500.00
TR4-H5253-1mg
¥23100.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    TNFRSF10D, CD264, TRAILR4, DCR2, TRUNDD

  • 表达区间及表达系统(Source)

    Human TRAIL R4 Protein, Fc Tag (TR4-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 56 - His 211 (Accession # Q9UBN6).

    Predicted N-terminus: Ala 56

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    TRAIL R4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 43.2 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    TRAIL R4 SDS-PAGE

    Human TRAIL R4 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    TRAIL R4 SEC-MALS

    The purity of Human TRAIL R4 Protein, Fc Tag (Cat. No. TR4-H5253) is more than 95% and the molecular weight of this protein is around 90-115 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     TRAIL R4 ELISA

    Immobilized Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) at 5 μg/mL (100 μL/well) can bind Human TRAIL R4 Protein, Fc Tag (Cat. No. TR4-H5253) with a linear range of 1-78 ng/mL (QC tested).

    Protocol

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背景介绍

Tumor necrosis factor receptor superfamily member 10D (TNFRSF10D) is also known as Decoy receptor 2 (DcR2), TNF-related apoptosis-inducing ligand receptor 4 (TRAIL receptor 4 or TRAIL-R4), CD264,which is the single-pass type I membrane protein. TNFRSF10D is receptor for the cytotoxic ligand TRAIL, which contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis.

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