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分子别名（Synonym）
TNFRSF10A,TRAIL-R1,CD261,APO2,DR4
表达区间及表达系统（Source）
Human TRAIL R1, His Tag (TR1-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Asn 239 (Accession # O00220-1).
Predicted N-terminus: Ala 24
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 24.8 kDa. The protein migrates as 20-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human TRAIL R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human TRAIL R1, His Tag (Cat. No. TR1-H5228) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 2-63 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human TRAIL R1, His Tag (Cat. No. TR1-H5228) on HIS1K Biosensor, can bind Human TRAIL with an affinity constant of 0.583 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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