Human TRAIL / TNFSF10 Protein, Fc Tag, active trimer (MALS verified)

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TRL-H5263-100ug
¥3500.00
TRL-H5263-1mg
¥23100.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Apo-2L,TRAIL,CD253,TNFSF10,Apo-2 ligand

  • 表达区间及表达系统(Source)

    Human TRAIL Protein, Fc Tag, active trimer (TRL-H5263) is expressed from human 293 cells (HEK293). It contains AA Val 114 - Gly 281 (Accession # P50591-1).

    Predicted N-terminus: Pro

  • 蛋白结构(Molecular Characterization)

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 86.4 kDa. The protein migrates as 90-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    TRAIL SDS-PAGE

    Human TRAIL Protein, Fc Tag, active trimer on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    TRAIL SEC-MALS

    The purity of Human TRAIL Protein, Fc Tag, active trimer (Cat. No. TRL-H5263) is more than 90% and the molecular weight of this protein is around 160-200 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     TRAIL ELISA

    Immobilized Human TRAIL Protein, Fc Tag, active trimer (Cat. No. TRL-H5263) at 5 μg/mL (100 μL/well) can bind Human TRAIL R3 protein, His Tag (Cat. No. TR3-H52H3) with a linear range of 5-39 ng/mL (QC tested).

    Protocol

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背景介绍

TRAIL, also known as TNFSF10, APO2L, Apo-2L, CD253, TL2, TRAIL, TNLG6A and TNF superfamily member 10. The TRAIL gene as a drug target, induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). DcR1 functions as a TRAIL-neutralizing decoy-receptor. The cytoplasmic domain of DcR2 is functional and activates NFkappaB. In cells expressing DcR2, TRAIL binding therefore activates NFkappaB, leading to transcription of genes known to antagonize the death signaling pathway and/or to promote inflammation.

前沿进展

 
药物研发进展
  • 英文全称:

    Tumor necrosis factor ligand superfamily member 10

  • 中文全称:

    肿瘤坏死因子配体超家族成员10

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    1 详情

  • 最高研发阶段:

    临床一期

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