Human TRAC Protein, His Tag

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TRC-H52H3-100ug
¥3675.00
TRC-H52H3-1mg
¥24255.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    TRAC

  • 表达区间及表达系统(Source)

    Human TRAC, His Tag (TRC-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 1 - Ser 115 (Accession # P01848-1).

    Predicted N-terminus: Ile 1

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    TRAC Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 14.8 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 0.2 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    TRAC SDS-PAGE

    Human TRAC, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • 活性(Bioactivity)-ELISA

     TRAC ELISA

    Immobilized Human TRAC, His Tag (Cat. No. TRC-H52H3) at 5 μg/mL (100 μL/well) can bind TRAC Antibody with a linear range of 0.2-5 ng/mL (Routinely tested).

    Protocol

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背景介绍

The transmembrane protein, TCR, comprise of two disulphide-linked polypeptide chains: a α and β chain, a γ and δ chain. Each polypeptide chain consists of a variable and a constant region. TRAC is the constant region of T-cell receptor (TCR) alpha chain. TRAC is presented on the surface of T cell and recognized peptide-major histocompatibility (MH) (pMH) that are displayed by antigen presenting cells (APC). TRAC is participate in adaptive immune response and has been well-studied in T cell therapy.

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