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分子别名（Synonym）
RANKL, CD254, TRANCE, OPGL, ODF
表达区间及表达系统（Source）
Rhesus macaque TNFSF11 Protein, His Tag (RAL-R5243) is expressed from human 293 cells (HEK293). It contains AA Gly 136 - Asp 317 (Accession # F7F5R8).
Predicted N-terminus: Gly 136
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 22.4 kDa. The protein migrates as 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Rhesus macaque TNFSF11 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Rhesus macaque TNFSF11 Protein, His Tag (Cat. No. RAL-R5243) was greater than 90% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Rhesus macaque TNFSF11 Protein, His Tag (Cat. No. RAL-R5243) at 1 μg/mL (100 μL/well) can bind Human Anti-TNFSF11, Human IgG1 with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Immobilized Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) at 1 μg/mL (100 μL/well) can bind Rhesus macaque TNFSF11 Protein, His Tag (Cat. No. RAL-R5243) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
Immobilized Human Anti-TNFSF11, Human IgG1 at 1 μg/mL (100 μL/well) can bind Rhesus macaque TNFSF11 Protein, His Tag (Cat. No. RAL-R5243) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
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