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分子别名（Synonym）
RANKL,CD254,TRANCE,OPGL,ODF
表达区间及表达系统（Source）
Cynomolgus TNFSF11 Protein, Fc Tag (RAL-C5263) is expressed from human 293 cells (HEK293). It contains AA Gly 136 - Asp 317 (Accession # A0A7N9DBU4).
Predicted N-terminus: Pro
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 46.9 kDa. The protein migrates as 55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Cynomolgus TNFSF11 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Cynomolgus TNFSF11 Protein, Fc Tag (Cat. No. RAL-C5263) at 2 μg/mL (100 μL/well) can bind Human RANK Protein, Mouse IgG2a Fc Tag (Cat. No. RAK-H5251) with a linear range of 0.05-0.8 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus TNFSF11 Protein, Fc Tag (Cat. No. RAL-C5263) at 2 μg/mL (100 μL/well) can bind Anti-TNFSF11 Antibody, Human IgG1 with a linear range of 0.01-3 ng/mL (Routinely tested).
Protocol
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