Biotinylated Human TMEFF2 Protein, His,Avitag™ (MALS verified)

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Qty.
TM2-H82E3-25ug
¥3675.00
TM2-H82E3-200ug
¥14490.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CT120.2, HPP1, TENB2, TPEF, TR, TR-2

  • 表达区间及表达系统(Source)

    Biotinylated Human TMEFF2 Protein, His,Avitag (TM2-H82E3) is expressed from human 293 cells (HEK293). It contains AA Phe 41 - Val 320 (Accession # Q9UIK5-1).

    Predicted N-terminus: Phe 41

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    TMEFF2 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 34.5 kDa. The protein migrates as 43-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    TMEFF2 SDS-PAGE

    Biotinylated Human TMEFF2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    TMEFF2 SEC-MALS

    The purity of Biotinylated Human TMEFF2 Protein, His,Avitag (Cat. No. TM2-H82E3) is more than 90% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     TMEFF2 ELISA

    Immobilized Human PDGF-AA Protein, premium grade (Cat. No. PDA-H5113) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TMEFF2 Protein, His,Avitag (Cat. No. TM2-H82E3) with a linear range of 2-39 ng/mL (QC tested).

    Protocol

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背景介绍

This gene encodes a member of the tomoregulin family of transmembrane proteins. This protein has been shown to function as both an oncogene and a tumor suppressor depending on the cellular context and may regulate prostate cancer cell invasion. Multiple soluble forms of this protein have been identified that arise from both an alternative splice variant and ectodomain shedding. Additionally, this gene has been found to be hypermethylated in multiple cancer types. Alternative splicing results in multiple transcript variants

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